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Pellucid dots5/8/2023 ![]() ![]() Mabberley, “Citrus (Rutaceae): A review of recent advances in etymology, systematics and medical applications,” Blumea-Biodiversity, Evolution and Biogeography of Plants, vol. Luvunga monophylla (DC.) Mabb, Australian Tropical Rainforest Plants, 8th Edition, CSIRO Publication,, 2020, (Accessed: 23 April 2020).ĭ. Parthasarathy, “Abundance and distribution of lianas in tropical lowland evergreen forest of Agumbe, Central Western Ghats, India,” Tropical Ecology, vol. Nesom, “Citrus trifoliata (Rutaceae): Review of biology and distribution in the USA,” Phytoneuron, vol. with special emphasis on microscopic technique,” Journal of Pharmacognosy and Phytotherapy, vol. Chauhan, “Pharmacognostic study of male leaves of Trichosanthes dioica Roxb. Ahmad, “Leaf, stem bark and fruit anatomy of Zanthoxylum Armatum dc.(Rutaceae),” Pakistan Journal of Botany, vol. Rezende et al., “Astra blue and basic fuchsin double staining of plant materials,” Biotechnic & histochemistry, vol. Johansen, “Plant microtechnique,” Plant microtechnique, 1940. Khandelwal, “Practical pharmacognosy techniques and experiments,” 19th edition, Mumbai, Pragati Books Pvt. Vatnick, “Environmental correlates of leaf stomata density,” Teaching issues and Experiments in Ecology, vol. ![]() Ash, “Manual of leaf architecture: morphological description and categorization of dicotyledonous and net-veined monocotyledonous angiosperms,” Smithsonian Institution, 1999.ī. Mabberley, “Australian Citreae with notes on other Aurantioideae (Rutaceae),” Telopea, vol. Zainudin Ibrahim et al., “On the vegetation and flora of Pulau Tioman, Peninsular Malaysia,” Raffles Bulletin of Zoology, vol. Navarro, “Citrus germplasm resources,” Citrus genetics, breeding and biotechnology, vol. Reece, “The botany of Citrus and orange relatives in the orange subfamily,” The Citrus Industry, vol. BR.(Saxifragales),” Journal of Scientific Research and Development, vol. Victoria, “Foliar Epidermal Morphology of Two West African Genera of Haloragaceae R. Dengler, “An integral part of botany,” American Journal of Botany, vol. Son, “Notes on the genus Paramignya: Phytochemistry and Biological activity,” Bulletin of Faculty of Pharmacy, Cairo University, vol. Kallunki, “Phylogeny of Rutaceae based on twononcoding regions from cpDNA,” American Journal of Botany, vol. ![]() Orrell et al., "Species 2000 & ITIS Catalogue of Life, 2014 Annual Checklist,” Species 2000: Naturalis, Leiden, the Netherlands, 2014. Mabberley, “Mabberley's plant-book: A portable dictionary of plants, their classifications and uses,” 3rd Edition, Cambridge University Press, 2008. The information obtained from this study will be helpful for the identification and future taxonomic-related studies of this plant species.ĭ. Calcium oxalates were present in the leaf and stem sections, while trichomes were detected in stem and petiole sections. Secretory cavities, xylem, phloem, and pericyclic fibers were found in the cross-sections of leaf, stem, and petiole. Pellucid dots were observed on both adaxial and abaxial leaf surfaces. Histochemical analyses were carried out using differential stains to identify the cellular components in leaf, stem and, petiole tissue sections. After infiltration, embedding and sectioning of the tissues were performed. The histological method was optimized by modifying the following parameters: number of fixation days, dehydration duration with degraded series of ethanol or butanol, clearing duration, and infiltration duration. Macroscopic characterization, optimization of histological procedure, and histochemical analyses of differential stains were carried out on the leaves, stems, and petioles of L. crassif olia leaves, stems, and petioles. Thus, the current study was aimed to investigate macroscopic and microscopic diagnostic features of L. Other than brief morphological descriptions, there are no published reports on other identification features of this plant. Luvunga crassifolia is an underutilized plant in the Citrus family. Institute of Biological Sciences, Faculty of Science, University of Malaya,ĭepartment of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaĬalcium oxalates, morphology, pellucid dots, secretory cavities Abstract Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia. ![]()
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